Abstract

Study of the EDA (+) fibronectin recognition structure on heparin. We carried out research into an absorber to remove EDA (+) fibronectin (EDA (+) FN) from the blood by using heparin. The elucidation of the EDA (+) FN recognition structure of the heparin is necessary for the development of the effective material. In this study, the examination of recognition structure for EDA (+) FN was carried out by using desulfate heparin and the heparin oligomer. The association constant (KA) of EDA (+) FN and the heparin decreased gradually (1.0E+8→1.0E+7M^-1) with desulfate treatment. There was hardly the change in KA with the decline of the molecular weight. As for the EDA (+) FN recognition structure of the heparin, our results showed that it was a low molecular weight level (less than MW=1.0E+3). We also found that the peculiar arrangement structure was not necessary.