Abstract
We have investigated an adsorbent which is able to both decrease endotoxin activity and adsorb cytokines in the blood. Several kinds of materials were tested for TNF-a adsorption in vitro. Marked TNF-α adsorption was observed in RCP160, which is a high porous strong acid cation exchange resin (Mitsubishi Chemical). Next, we examined this resin's adsorption characteristics for other cytokines and its effect on endotoxin activity (Limulus gelation activity and ability to produce cytokines). This resin showed adsorption for other inflammatory cytokines such as IL-1β, IL-6 and IL-8. The Limulus gelation activity of endotoxins decreased after treatment with this resin. The production of TNF-α, IL-β and IL-6 by endotoxin-stimulated blood cells also decreased after treatment with this resin. These results suggest that RCP160 has potential to be a useful adsorbent in the treatment of not only Gram-negative sepsis but also other diseases with hypercytokinemia.
