Abstract
This study represents the experimental efforts directed to the development of vascular suture material which possesses the thromboresistant qualities.
For this purpose, we prepared the nylon suture material immobilized urokinase by linking the urokinase to 7-0 nylon fiber.
The procedure of immobilization was shown in Figure 1. Urokinase was linked to nylon through the grafted copolymer such as polyethyleneimine and gantrez (malefic anhydride/methylvinyl ether copolymer) by peptide linking.
The fibrinolytic activity of this material was measured by the standard fibrin plate.
These results were as follows:
1. This material was stable on storage at 4°C for over six months.
2. This material lost about 50% of initial fibrinolytic activity following a few repeated fibrinolysis test but retained 30-20% activity.
3. This material was influenced in fibrinolytic activity by added serum.
4. This material kept the favorable fibrinolytic activity after the procedure of disinfection.
5. This material showed the favorable antithrombotic action in the experiment of rabbit.
The nylon suture material immobilized urokinase might be able to put to the practical utility in vascular surgery.
