Abstract
Effect of dilution-substitution on solutes clearance in hemofiltration were investigated, since dilution of the serum is known to reduced protein resistance to hemofiltration and is expected to release the small to middle molecules from the protein-bound condition.
Experimental results evidenced: I) Clearance values of middle molecules presented on gel-chromatograms of uremic sera were very similar to those of urea and creatinine, and were not much affected by twice to ten times dilution with electrolytes solution. 2) Sieving coefficient of each solute with molecular weight from 60 to 5000 were unchanged, even when serum protein concentration was reduced to half by dilution.
Conclusion is made that the uremic middle molecules will not be bound to protein, and substitution of plasma volume is much more significant compared to dilution of serum in hemofiltration.
