Abstract
Continuous hypothermic perfusion is today an accepted method of renal preservation in the world. Some preservation machine such as Belzer, Waters (Mox 100), Travenol and Gambro are clinically used in U. S. A. and Europe. However, perfusion method of kidney preservation is not yet clinically used in this country. We have been developing a new preserving machine that would be easier to handle and cheaper to purchase. This machine is consisted of an organ chamber, two roller pumps, a membrane oxygenator, a heart exchanger, a refrigerator, two in-line thermistor probes and two automatic pressure controllers. An organ chamber has double walls which is consisted of inside and outside walls. Cold water flows between two walls to cool an organ chamber. A pressure controller maintains arterial systolic pressure to the indicated one, which is usually 60mmHg, and when the pressure rises, the controller decreases the rotation of the roller pump, making the renal flow rate lower. Belzer's cryoprecipitated plasma was used as the perfusate and we succeeded in preserving canine kidneys using this machine for 72-96 hrs.
