HEPATIC ASSIST SYSTEM WITH SEPARATED HEPATOCYTE FROM RABBIT

Abstract

We mad is chemic liver failure model in, dogs with two step method, first postcaval shunt and second ligation of hepatic artery and common bile duct.
Hepatic assist was performed by separation rabbit liver cells (Hepatocyte). Both hepatic failure groups were by infusion of 10% glucose solution meintained and Linger's solution in the proportion one to two, and then this prognosis of hepatic assist group (three dogs) were compared to control group (seven dogs).
Chamber priming volume was 85ml and the blood was contacted with hepatocyte through N. M. F. There was no significant difference between bouth group in survival time but there was significant decrease of serum NH₃-N, GPT and LDH in hepatic assist group.