Abstract
The formation of an insoluble fibrin network plays an important role in the process of wound healing. To form such an insoluble fibrin, thrombin, a blood clotting factor XIII (F XIII) and Ca++ are required. For the enhancement of local fibrin accumulation, we immobilized thrombin and F XIII on absorbable gelatin sponge and also on some suture materials using our newly devised technique.
Basic studies on the degree of fibrin formation on the materials both in vitro and in vivo showed that a large amount of fibrin had formed, Even in tissue liquid containing less fibrinogen than plasma, remarkable fibrin network had formed. Moreover, the fibrin network formed in vivo adapted well with the tissues. On the other hand, no fibrin formed on the control materials.
Clinically, the immobilized suture materials were used for skin suture and the extraction force to take out the sutures was measured by tension meter on the 8th postoperative day. The result suggest that much fibrin formed and the fibrin network adapted well with patients subcutaneous tissue.
As a model of embolus formation in vessels, we used Chandlers roatating tube method, In this tube, large embolus formed on the immobilized sponge in a moment. So, we utilized the sponge as a thrombogenic material for transcatheter embolization. The embolization was successful and 2 monthes after, no recanalization could be seen.
We are going to utilize these for promotion of wound healing, protection of anastomosis, transcatheter embolization and so on.
