2005 Volume 17 Issue 1+2 Pages 49-54
The advent of electronic noses opened a new kind of analytical approach, which offers an easy, quick, and cheap measurement comparing to conventional analytical approaches. It has potential applications on many different fields, such as assessment of various foodstuffs and beverages, environmental contamination monitoring and medical diagnostics etc. In this study, we used odorant binding protein (OBP) as odor-sensing material for developing such kind of artificial odorant biosensor. And biological films of OBP-1F (rat OBP) were deposited by Langmuir-Blodgett (LB) technique on gold electrode previously functionalised with 1-octadecanethiol (ODT) by self-assembled monolayers (SAMs). It is known that successful immobilization of the Langmuir-Blodgett films depends strongly on the characteristics, particularly stability, of the corresponding Langmuir films at the air/water interface. Hence the characteristics of monolayer of amphiphile octadecylamine (ODA) and the mixed monolayer of ODA/OBP were studied. Electrochemical impedance spectroscopy (EIS) was used to monitor the response of the system to a specific odorant molecule, isoamyl acetate.