Abstract
A fiber optic bio-sniffer for high-selective alcohol vapor monitoring with an UV-LED was constructed and tested. Since many kinds of chemical components are transpired from human bodies, high-selective gas sensor is strongly requested. In order to achieve high selectivity, the sensor utilizes fluorescence of nicotineamide adenine dinucleotide (NADH) (491nm), which is produced by the enzymatic reaction of alcohol dehydrogenase (ADH). The bio-sniffer was fabricated by attaching a wet enzyme immobilized membrane to the fiber-optic NADH measurement system. The fluorescent intensity significantly increased with a stable value related to the ethanol gas concentration when ethanol vapor was exposed.
