Fiber-optic chemifluorescent immunoassay for house dust mite allergen Der f1

Abstract

A fiber-optic chemifluorescent immunoassay system for detection of Der f1 was constructed. Der f1 is one of the most important indoor allergens derived from Dermatophagoides farinae. The system measures Der f1 as fluorescence amplified by an enzymatic reaction between labeled enzyme to a detection antibody and fluorescent substrate. The measurement range for Der f1 was from 0.49 to 62.5 ng/ml which was equal to that of the enzyme-linked immunosorbent assay (ELISA). The system showed also high selectivity as the result of the comparison of the sensor outputs between Der f1 and other airborne allergens.