Abstract
In order to reconstruct a three dimensional image such as MRI, CT and confocal image from series of cross-sectional images, it was desirable to set an objective threshold at each x-y image and along z-axis. However it was not so easy to set the adequate threshold from noisy or low contrast images. In this case it sometimes used to set the threshold manually by experienced eye. The manual operation was restricted in case of the numerous data. In this study, we applied the algorithm developed by Otsu (DTSM) for the selection of the objective threshold in combination with the algorithm of Maximum Intensity Projection (MIP) and/or Average Intensity Projection(AIP). As a result the combination of AIP-DTSM gave the reasonable threshold to provide the good 3-D images.
