Japanese Journal of Breeding
Online ISSN : 2185-291X
Print ISSN : 0536-3683
ISSN-L : 0536-3683
Histochemical studies on the cytoplasmic male-sterility of sugar beets, with special reference to the hydroeenion concentration, carbohydrates and amino acids in anthers.
Sadaji HOSOKAWAChikahiro TSUDATakeo TAKEDA
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1963 Volume 13 Issue 2 Pages 117-124

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Abstract
The present paper is devoted to the observation of histochemical phenomena in anther tissue of cytoplasmic maie-sterile sugar beets contrasted with those in normal strains. I. Hydrogen-ion concentration in anther tissue. Based on several observations using pH indicators (Methyl red or Brom thymol blue), following conclusions could be drawn(Table 1, 2, 3 and Fig. 1, 2, 3). In the early stage of microsporogenesis, the pH values in anther tissue of both rnormal and malesterile types are rather high ranging: from 6.6 to 7.0. As the age of anthers of normal types advances to the microspore stage, the pH values decrease to about 6.2, then they increase agnin to about 6.8 at the D. ollen-grrain stage, and, at the anthesis, the pH values decrease to about 6.2. On the other hand, the pH values observed in anthers of male-sterile types never show such zig-zag curves as those of normal strains during the microsporogenesis, but gradually decrease to about 6.2 at the anthesis.
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© Japanese Society of Breeding
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