Japanese Journal of Breeding
Online ISSN : 2185-291X
Print ISSN : 0536-3683
ISSN-L : 0536-3683
IN VITRO CULTURE OF EXCISED OVULES OF WHITE CLOVER, TRIFOLIUM REPENS L.
Tetsuo NakajimaYasuteru DoyamaHiroshi Matsumoto
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1969 Volume 19 Issue 5 Pages 373-378

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Abstract
Many of the ovules excised 4∼5 days after anthesis could develop to mature seeds on Nitsch's medium containing yeast extract and NAA. On the othef hand, some mature seeds were obtained from the young ovules excised immediately after fertilization, only when they were cultured on the medium supplemented by fruit juice prepared from young fruit of cucumber or water melon.
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© Japanese Society of Breeding
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