Abstract
In previous paper it was reported that pollen grains carrying B gene, black glume color, in two-rowed barley were selected with some ratio by the high temperature treatment of its F1 spiklets (Bb in genotype). This was re-examined in other combinations of varietal crossings with several marker genes. It was proved that segregation ratios of genes Bb and Trdtrd (third outer glume) belonging to the 5th linkage group were affected by high temperature but genes on the other linkage groups were not affected.