Abstract
Ufeit-type electrophoretic apparatus for polyacrylamide gel-columns (U-type) was devised and the procedure of isoelectric focusing was described. The procedure for peroxidase zymograrns of rice plant was noted under thirty-six conditions different in voltage, running time and concentration of electrode solutions. The condition that developed the best zymograms over the whole length of gel-column was taken as the optimum condition as follows: gel-column 3mmx150mm long, 10V/cm for voltage, 16-18hrs. for running time and 0. 8% V/V(P)∼2. 2% V/V(T) or 1. 2% V/V(P)∼3.3% V/V(T) for electrode solutions.Ufeit-type electrophoretic apparatus for polyacrylamide gel-columns (U-type) was devised and the procedure of isoelectric focusing was described. The procedure for peroxidase zymograrns of rice plant was noted under thirty-six conditions different in voltage, running time and concentration of electrode solutions. The condition that developed the best zymograms over the whole length of gel-column was taken as the optimum condition as follows: gel-column 3mmx150mm long, 10V/cm for voltage, 16-18hrs. for running time and 0. 8% V/V(P)∼2. 2% V/V(T) or 1. 2% V/V(P)∼3.3% V/V(T) for electrode solutions.
