Abstract
The abilltles of callus Inductron and organogenesls from cultuled noral organs of some crucifcrous plants were irrvestigated using a revised MURASHIGE and SKOOG (1962) medium with various combinations of plant growth regulators. Best organogenesis was achleved cultunng floral organs except anther, with 6-benzyladenosine (6-BAR) and Indole-3-acetic acid (IAA) at 10-5M. Floral organs cultured :[n medium supplemented with 2, 4-dichlorophenoxyacetic acid (2, 4-D) alone or with 6-BAR both at l0-5M gave the best callus induction but no organogenesis. Floral organs cultured from buds at the pollen tetrad to uninucleated pollen stage gave higher percentagre of callus induction and organ differentiation than other developmental stages. Petal showed rapid organogenesis while anther proved refractory. There was rapid growth and development of shoots or plantlets regnerated from floral disc explant. Pretreatment of 6-BAR to the inflorescence of cauliflower and Italian broccoli 24 hours before inoculation favored callus induction in anthers. Floral organs diffcred in callus induction and organogeriesis in diffenent plant species. Within Brassica oleracea L., caulifower. Italian broccoli and flowering kale were similar in callus induction and organogenesis while cabbage was slower to differentiate organs, Brassica and Orycho-phragmus were found to be similar In organogenesis ability; Raphanus was the most difiicult genus in orgranogenesis.
