Estimation of Genetic Homogeneity by Isozymes from Interspecific Hybrids of Vicia :II Amylase Isozyme Patterns in the Hybrid Progenies between Vicia amphicarpa, true and V. macrocarpa

Abstract

In order to estimate the degree of genetic recombinate on and genetic homogeneity in the interspecific hybrid progenies between Vicia alephicarpa, true (female) and V. macrocarpa (male), amylase isozyme patterns were analyzed by disc electrophoresis. Each parental species has three amylase bands, of which two ai-amylase (a₁ and a₂ band) were common, but one B (B₁ or B₂) was not. B₁ band was detected in male and B₂ in female parent. Both F₁ and F₂ Showed fcur bands derived froni the parents. Zymograms detected in the F₃ and F₄ were assembled in ten groups according to the pattern. These groups were characterized by the presence or absence of both parental bands. The frequency of male parental-band (type I) was higher in F₄ than F₃, and that of F₁ band (type VIII) was lower in F₄