Abstract
To determine the basic medium suitable for embryo culture, the growth response of embryos to different cultural condition was examined in Japanese garden iris, Iris ensata. Embryos which were isolated from ovules at about 60 days after pollination were explanted onto various cultural media (Table 1) in test tubes under aseptic condition, and were grown at about 20°C under continuous illumination. Sixty days after inoculation, germination rate, dry weight, plant height and root length were examined, and the followlng results were obtained. Nitsch's inorganic medium containing 2% sucrose and O.4-0.6% agar and being ad justed at pH 4-5 was the best as the basic medium for embryo culture of I. ensata. In all the supplements added to the basic medium, 30 ppm peptone and 5ppm gibberellin were the most effective for the growth and the germination of embryos, respectively, but these additions were not necessarily essential to embryo culture.
