Abstract
In wheat (Triticum aestivum L.) a successful chromosome doubling of haploids (2n=21) is essential for the production of homozygous plants, following the intergeneric cross with tetraploid Hordeum bulbosum L. and the in vitro culture of immature haploid embryos. The effects of colchicine treatment on haploid wheat plants at different developmental stages were investigated. The result showed that the haploid plants treated at the 2- to 3-tiller stage with 0.10% colchicine solution produced seeds at frequency of 95.6% of treated plants.
