Abstract
This study was conducted to obtain information on the causes of variation of anther-derived doubled haploid lines from a inbred cultivar of tobacco (Nicotiana tabacum). From a spontaneous doubled haploid lines of androgenetic origin of tobacco, an experimental series of doubled haploid lines was raised through anther culture or induced parthenogenesis. The field test of 38 anther-derived and 12 ovule-derived doubled lines along with 17 lines of the selfed parent revealed considerable variations among the anthel-derived lines both in quantitative and qualitative characters. On the other hand, all the ovule-derived lines, some of which were doubled by colchicine treatment while the others were doubled spontaneously, were not different from the selfed lines of the parental plant for the characters measured. No cytoplasmic effects were detected in the reciprocal crosses between anther-derived doubled haploid lines possessing variations and the parental line. These results indicate that the variations observed among doubled haploid lines of androgenetic origin were due to the mutations of nuclear genes in the course of androgenesis.
