Abstract
Percentage of successful pod setting in the intersubgeneric hybridization between G.max(2n=40, maternal) and G.tomentella(2n=80, paternal) was estimated to be 2.5 to 7.6% Germinability of the G.tomentella pollens of G.max stigmas, elongation of the pollen tube in the style and arrival of the pollen-tube tips at the ovules were detected by SEM and fluorescence microscopy. Aneuploidy (2n=64) of the hybrid embryo cells and peroxidase zymogram pattern specific to the hybrid-derived calli were observed by light microscopy (smear method) and polyacrylamide gel electrophoresis, respectivly. Moreover, histlogecal observation of the hybrid embryo was carried out to investigate the extant of the hybrid embryo growth. It was concluded that morphologically normal growth may continue at least until heart or a more advanced developmental stage, but markedly retarded growth accompanies it and eventually it ceases growing and death of the hybrid embryo results. Though, we couldn't get intact hybrid plant, all of the facts revealed proved formation of the genuine hybyid embryos between G.max and G.tomentella.
