Abstract
Tissue cultures of wheat (Triticum aesticum L., cv. Norin 61, 2n=6x=42) were initiated from the immature haploid embryos which were produced by the intergeneric crosses with Hordeum bulbosum L. Nine immature embryos ca. 0.5mm in diam which were cultured for fifteen weeks on GAMBORG'S B5 medium supplemented with 2ppm of 2, 4-dichlorophenoxyacetic acid, developed to calli ca. 15 mm in diam. The calli were individually divided into ten small pieces and then transferred onto a hormone-free B5 medium for plant regeneration. A total of 63 green plants were regenerated. All the regenerated plants were euhaploids with 21 chromosomes.
