Japanese Journal of Breeding
Online ISSN : 2185-291X
Print ISSN : 0536-3683
ISSN-L : 0536-3683
Somatic Embryogenesis and Plant Regeneration from Suspension Callus Culture in Melon (Cucumis melo L.)
Toshiro ORIDATEKatsuji OOSAWA
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Keywords: plant regeneration
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1986 Volume 36 Issue 4 Pages 424-428

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Abstract
Somatic embryos and plantlets were induced from suspension culture of cotyledon callus in melon (Cucumis melo L.). Cotyledons excised directly from bared mature seed were cultured on MS agar media with several concentrations of 2, 4-D and BA. Three weeks old calli were transferred to MS liquid medium without growth regulators, and cultured on a reciprocal and gyratory shaker (115 rpm). Somatic embryos and plantlets were observed within 2-3 weeks in the liquid media. The calli cultured on MS agar medium with 1.0mg/l 2, 4-D+0.1mg/l BA were the most suitable for embryogenesis.
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© Japanese Society of Breeding
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