Abstract
Protoplasts of L.pimpinellifolium were isolated from cotyledons from about 10 day old seedlings which were grown under sterile conditions at 20°C and 4, 500 lux. Isolated protoplasts were cultured in a plating medium of TM-2 (SHAHIN, 1985) with liquid on agarose and, subsequently, on the second medium based on TM-3 (SHAHIN, 1985) under the conditions of 25°C and 400 lux. The calli brought up to about 1.0 mm calli were transferred onto the shoot induction medium of TM-4 (SHAHIN, 1985) where shoots never appeared. Calli were again transferred to modified MS medium (MURASHIGE and SKOOG, 1962) supplemented with ZR (Zeatin riboside) on which shoots were regenerated at the rate of 27% during the ten day culture from 45 days after plating of proto plasts.
