Abstract
Immature barley embryos in culture show a high ability of plant regeneration, but the period in which embryos can be used is limited. To overcome this difiiculty, regeneration of shoots from callus cultures derived from seeds and mature embryos detached from seeds was attempted, using 16 six-rowed and 4 two-rowed varieties of barley. Callus was initiated from seeds and mature embryos cultured on B 5 medium with 2, 4-D at concentrations ranging from 5 to 100μM and then allowed to grow on the same medium for two months. Regeneration of plants was observed in two varieties Golden Grain and Haisa 4X when 2, 4-D at concentrations of 10 and 30μM was used for the culture of calli derived from seeds and in one variety Golden Promise at concentrations of 10 and 15μM for the culture of calli derived from mature embryos. It must be noted that regeneration of plants occurred on medium with high concentrations of 2, 4-D.
