Abstract
Barley varieties classified into three isozyme patterns by isoelectric-focusing of high pla-amylase were crossed. F2 and F3 segregation for the banding patterns of a-amylase indicated that the three types showed basically co-dominant relationships. In the cross-combination of type lxtype 2 ('Haruna NijoxKlages' and 'Haruna Nijox Clipper'), new banding patterns were observed with a low frequency. It was estimated that the gene coding for the band detected only in type I was transferred from type l to type 2 with the genetic recombination. The estimates of the recombination values were 5.3:tl.8% ('Haruna NijoxKlages') and 9.7:i:3.1% ('Haruna NijoxClipper'), respectively. F3 segregation for (x-amylase activity in germinating seeds was also examin" ed. The results revealed that the differences in the structural genes were not definitely related to the activity of a-amylase, and that the variety Haruna Nijo harboured a few genes with dominant effects relating to a-amylase activity.
