Japanese Journal of Breeding
Online ISSN : 2185-291X
Print ISSN : 0536-3683
ISSN-L : 0536-3683
Plant Regeneration from Dried Callus of Carrot (Daucus carota L.)
Noboru KAIMORINorindo TAKAHASHI
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Keywords: totipotency
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1989 Volume 39 Issue 3 Pages 379-382

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Abstract
Callus of carrot (Daucus carota L.) was dried for about 6 hours at 27°C in tall petri dishes containing silica gel. The water content of dried calli was around 3%. After storage for four days at 27°C in the dry state, they were transferred directly to MURASHIGE and SKOOG's agar medium (MURASHIGE and SKOOG 1962). Survival rate was as high as 90% as determined by callus growth and embryo and plantlet differentiation in 21-day cultures. The significance of the successful retention of differentiation potentials in dried callus is discussed in relation to conservation of plant genetic resources.
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