Abstract
A simple and efficient method for identification of hybrids after cell fusion was established by combining a method for the efficient extraction of DNA from very small amounts of plant tissue and Southern blotting with non-radioactively labeled rDNA from rice as a probe. One hundred milligrams of leaf or callus tissue, are sufficient for identification of the hybrid, and the amount of material can be reduced to as little as 10 mg. Since this method permits selection of parasexual hybrids not only at the plantlet stage but also at the callus stage, hybrids can be identified at the early stages of the development of the products of cell fusion.
