Hybrid Cell Lines Produced by Protoplast Fusion between Luffa cylnidrica

Abstract

Hybrid cell lines were produced by the intergeneric protoplast fusion between Luffa cylindrica ROEM. (L. cylindrica) and Gynostemma pentaphyllum MAKlNO (G, pentaphyllum). Protoplasts derived from the cotyledons of L. cylindrica and protoplasts derived from the cultured cells of G. pentaphyllum were fused according to the method of MENCZEL and WOLFE. Four fusion products were confirmed as hybrid cells based on isozyme analysis