Restriction Fragment Length Polymorphisms of Grapevine DNA with Phenylalanine Ammonia-Lyase cDNA

Nami YAMAMOTO; Genki ONO; Kunio TAKASHIMA; Akira YOTSUKA

doi:10.1270/jsbbs1951.41.365

Japanese Journal of Breeding

Online ISSN : 2185-291X
Print ISSN : 0536-3683
ISSN-L : 0536-3683

Restriction Fragment Length Polymorphisms of Grapevine DNA with Phenylalanine Ammonia-Lyase cDNA

Nami YAMAMOTO, Genki ONO, Kunio TAKASHIMA, Akira YOTSUKA

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Keywords: Vitis vinefera,Vitis labrusca,grapevine,RFLPs,DNA extraction,phylogeny,L-phenylalanine ammonia-lyase

JOURNAL FREE ACCESS

1991 Volume 41 Issue 2 Pages 365-368

DOI https://doi.org/10.1270/jsbbs1951.41.365

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Abstract

Total DNA was extracted from leaves of grapevines, 8 cultrvars of Vitis vinifera, Concord (V. labrusca) and a rootstock variety, F-3309. All the tested DNA showed different Southern analysis patterns using L-phenylalanine ammonia-lyase (PAL) cDNA of carrot (Daucus carota) as a probe. A dendrogram based on the RFLPs of PAL cDNA relatively agreed well with morphological taxonomy.

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