Identification of Ploidy Level of the Regenerated Plants by Anther Culture in Rice

Abstract

In the regenerated plants by the anther culture of rice, panicle length, plant height and glume length were measured. Only the glume length was able to separate regenerated plants into three distinct classes.The cytological analysis of these plants revealed that the three classes based on the glume length consisted of haploids, diploids and triploids, respectively. Similar results were obtained in the regenerated plants derived from irradiated callus. With irradiation as a physiological disorder, the glume length was a stable index of the ploidy level of rice plants.