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Japanese Journal of Breeding
Vol. 45 (1995) No. 1 P 71-74

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http://doi.org/10.1270/jsbbs1951.45.71


A method for Agrobacterium -mediated transformation of torenia (Scrophulariaceae) is reported. Leaf segments of torenia plants grown in vitro were infected with the Agrobacterium strain LBA4404 harboring a binary vector containing the neomycin phosphotransferase IIgene, P -glucuronidase (GUS) gene lvith an intron and hygromycin phosphotransferase gene. The infected explants were cultured on Murashige and Skoog medium supplemented with 1 mg/l benzyladenine, 100 mgfl carbenicillin and 300 mg/l kanamycin (selection medium) for regeneration. Twelve shoots out of 67 shoots which regenerated from green compact calli on the selection medium were GLTS-positive. Polymerase chain reaction analysis confirmed the presence of the GUS gene in the GUS-positive plants. Mendelian inheritance of the GUS activity in the progenies of the transformed plants was demonstrated.

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