Plant Regeneration from Immature Seed-Derived Callus of Cypripedium macranthos Swartz var. taiwanianum (Masamune) F. Maekawa

Abstract

To obtain basic information for the micropropagation and future breeding of endangered Cypripedium species, plant regeneration from immature seed-derived callus of Cypripedium macranthas var. taiwanianum was studied. Calli, induced from immature seeds on T medium (Tsutsui and Tomita 1990) supplernented with 200 mg/l yeast extract, 10 g/l sucrose and 2 g/l gellan gum, were used for the plant regeneration test. The regeneration was observed in three lines of callus on media containing 10^-5M NAA with or without the addition of 10^-6M BAP. After vernalization, regenerated plants were established.