Abstract
Callus induction and morphogenesis from different black-gram explants were tested on MS basal medium supplemented with B5 vitamins, IAA, NAA, IBA, KIN and BAP individually and in combinations. The explants were hypocotyl, epicotyl, axillary bud, cotyledonary node and immature leaf. The optimal levels of the frequency of callus induction was 22.8μM of IAA or 16.1μM NAA and in combination with 2.2μM of BAP. Among the seedling explants, hypocotyl was found to be more efficient in producing callus. Shoots were induced from callus cultures of hypocotyls, epicotyls, axillary bud, cotyledonary node and immature leaf with varying frequencies in the medium containing KlN (2.3-9.3μM) or BAP (2.2-8.8μM) and in combination with IAA (2.8μM) or NAA (2.6μM). Multiple shoots were obtained using cotyledonary node segments. The regenerated shoots rooted best on MS basal medium containing 9.8μM IBA. Seventy three per cent of the shoots produced roots, and 80-85% of the plantlets survived under greenhouse condition.
