Abstract
Field performance of the progeny of protoplast-derived barley plants (cvs. Igri and Dissa) was investigated. The least significant differences for five agronomic characteristics (culm length, heading date, fertility, spike length and spikelet density) were calculated to compare the field performance of the protoplast-derived plants with that of control plants. Our results indicated that most of the progeny plants showed no abnormal traits and set seeds, but the field performance of those plants changed in some agronomic characteristics. These facts suggest that we should make an effort to establish in vitro culture conditions which are less mutagenic to cultured cells of barley.
