Abstract
1. In this paper the writers deal with a survey of chlorophylls and carotenoids in rice plants by means of paper chromatography, using 20 materials of cultivated strains and mutant lines. 2. The extractioh of leaves was made with a liquid (methanol : acetone=3:1 solution). And the ascending development of spot area carried out 40 minutes with a solution (toluol : petroleum benzine =2:1) in a chamber regulated at 1-2 'C. Thus 5 coloured areas were separated in order of chlorohyll b (yellowish green), chlorophyll a (blue gleen), two xanthophylls (yellow) and carotene (orange yellow) frcm the original point (Fig. 1). 3. Although the Rf value of carotene pigment was invariably having no relation to the amount of spot sample, but others increased according to those amounts. However, in this present experiment, the coloured areas in all the pigments in question increased rectilinearly depending upon the amount of spot sample (10-120 r)Consequently this method seemed to be used for the quantltatrve analysis of leaf pigments (Tabs. l-3, Figs.2-3).
