Development of a model of accelerated blood coagulation in a dialysis circuit using bovine blood

Abstract

In continuous renal replacement therapy, rapid detection of intracircuit clotting is a major challenge. In this study, we aimed to establish a model of accelerated clotting that can simulate intracircuit clotting in a short period. The arterial circuit was infused with citric acid as an anticoagulant, which was removed through dialysis to promote clotting in the venous chamber. By estimating the citric acid concentrations in both the arterial and venous circuits using a single-compartment model, we manipulated the citric acid concentration in bovine blood and carefully observed clot formation in the circuit. We achieved clot formation in the venous chamber with a venous citrate concentration of 5.1 mM after 129 min by maintaining an arterial citrate concentration in the range of 10.0-12.0 mM.