2012 Volume 3 Issue 1 Pages 40-45
Background: It has been reported that a PMMA membrane hemofilter (hemofeel®CH-1.0N) provides intense adsorptive removal capacity for interleukin 6 (IL-6). We assessed in vitro the ability of hemofeel®CH-1.0N to adsorb the high concentration of IL-6 (477,000pg/mL) from human waste plasma, by CHD or CHF. Methods: Under the plasma flow rate of 80mL/min, the body fluid removal rate of 0mL/hr, and the dialysate/filtration flow rate of 400mL/hr, we assessed using ELISA method for the IL-6 concentrations, IL-6 clearance, IL-6 SC, IL-6 removal rate, total amount of removed IL-6, and assessed TMP in the waste plasma collected from the inlet and the outlet of the hemofilter, and from the filtered solution. Results: The IL-6 concentrations on the inlet side, at 0.5, 6, 18 and 48 hrs after the initiation of CHD/CHF, were 186,000/161,000, 11,600/6,650, 1,440/564, 1,040/546 respectively. The IL-6 clearances, at 0.5 and 18 hr after initiation of CHD/CHF, were 31/36 and 2/2 mL/min, respectively. IL-6 was removed at a rate of approximately 97% from the plasma within 6 hrs. Conclusions: The total amount of IL-6 removed was 4 hundred million pg for both CHD and CHF. To ascertain the limits of the adsorption capacity, it is necessary to perform the spiked blank recovery test.
