2013 Volume 4 Issue 2 Pages 128-132
High mobility group box 1 (HMGB1) is released from various types of cells and is a pro-inflammatory ligand of Toll-like receptors and receptors for advanced glycation end products (RAGE). It is known that HMGB1 accelerates inflammation during sepsis and disseminated intravascular coagulation (DIC). Polymyxin B immobilized fiber column direct hemoperfusion (PMX-DHP) is an effective therapeutic modality for sepsis by directly adsorbing endotoxin. However, whether or not PMX-DHP directly adsorbs HMGB1 remains controversial. In order to answer this question, we measured time-dependent and column-dependent changes in serum HMGB1 concentration in the clinical setting. However, we did not see any significant reduction in serum HMGB1 level. Next, we investigated the effects of polymyxin B-immobilized fiber on HMGB1 concentration in experimental HMGB1 solution in vitro. Consistent with the clinical data, there was no significant adsorption of HMGB1. We conclude that PMX-DHP does not directly reduce the blood HMGB1 level during treatment. Its remote effect may play an important role in regulating HMGB1.
