Molecular Science for Analyzing Dynamics of Biomolecules in Living Cells

Abstract

Developments in methodological and technological aspects for biomolecular imaging are highly expected. We developed a novel design of split GFP and split luciferase; the principle is based on reconstitution of the split-reporter fragments when they are brought sufficiently close together. The usefulness of the split-reporter reconstitution has been demonstrated such as imaging of endogenous mRNA, protease activities, protein-protein interactions and protein transport into a specific organelle. This review introduces the basic principle of the reconstitution technology and its wider application in the fields of biology and basic medicine.