Abstract
Two fractions having thymidine kinase (ATP; thymidine 5 phosphotransferase, EC 2. 7. 1. 21) activity were separated from tumor cells such as Yoshida sarcoma, Ehrlich ascites tumor, Hepatoma AH 130, Morris hepatoma 7793 and Morris hepatoma 7794 A by means of DEAE-cellulose column chromatography, while only a single fraction of thymidine kinase was observed in normal cells such as bone marrow, spleen, embryonic and regenerating rat liver.
The properties of thymidine kinases from Yoshida sarcoma were compared with those of the regenerating liver thymidine kinase.
By the properties we meant Km for thymidine and for ATP, heat stability, urea effect and optimal pH.
DEAE-Cellulose column chromatography of purified thymidine kinase from the Yoshida sarcoma yields two peaks of enzyme activity.
Molecular weight of Peak I and Peak II have been estimated to be 70,000 and 120,000, respectively.
