Abstract
In order to evaluate the plasma LPL activity after intravenous administration of heparin, the authors established a new method determining FFA which was liberated through LPL by modified Ui-Itaya's colorimetric method by using Intralipid activated by human serum as the substrate.
The optimal conditions for this enzyme reaction with regard to Intralipid concentration, albumin concentration, human serum concentration, pH, temperature and time course were evaluated. The LPL activity showed the correlation to the volume of post-heparin plasma. 1 M sodium chloride showed inhibition of 40. 5% on lipolysis in post-heparin plasma.
Precision of this method showed a coefficient of variation of±5.0%.
