Abstract
Studies were carried out on the urinary excretion and biosynthetic pathways of aminoacetophenone derivatives.
1. Administration of tryptophan to hens or rats resulted in occurrence of aminoacetophenone (AAP), 2-amino-3-hydroxyacetophenone (3 HAAP) and 2-amino- 5-hydroxyacetophenone (5 HAAP).
2. Kynurenine was enzymically converted to kynurenamine by 0.01 M phosphate buffer (pH 8. 0) extract of rat liver and then non enzymically to AAP.
3. 5HAAP was formed from 5-hydroxykynurenine via 5-hydroxykynurenamine by 0.01 M phosphate buffer (pH 8. 0) extract of rat small intestine through the similar route to that of formation of AAP from kynurenine.
4. Rat liver rnicrosomes catalyzed hydroxylation of AAP to yield 3HAAP in the presence of NADPH.
