Abstract
For analysis of creatine kinase (CK) isoenzymes, the original method according to Van der Ween et al. was modified, and an improved agar gel electrophoresis with a good sensitivity was brought in use.
CK isoenzymes were investigated on sera from patients with muscular dystrophies (47), polymyositis (4), myocardial infarction (6) and cerebral hemorrhage (3).
In 47 cases of progressive muscular dystrophies (32 Duchenne, 5 benign Duchenne, 6 congenital and 4 limb-girdle type), the relation between the serum total CK activity and age of the patients in each type showed the same tendency, i. e. a higher activity in the earlier stage, as previously reported by other authors. In the congenital type, the total CK activity elevated not so much as in the Duchenne type, however, the ratio of MB isoenzyme fraction was higher than in the latter. In 4 cases of polymyositis with a high serum total CK, the activity decreased rapidly after steroid therapy, while the MB isoenzyme fraction maintained a relatively high level.
At the peak stage in acute myocardial infarction, serum MB isoenzyme was detected independently of the total CK activity, and disappeared within about 80 hours.
In 3 cases of cerebral hemorrhage with a high serum total CK, isoenzyme analysis revealed no BB but MM fraction which is considered to be of the muscular origin.
The BB isoenzyme fraction was not detected in this series except in one sample from fluid contents of a cystic brain tumor.
