Abstract
1. Affinity chromatography was effectively applied to the analysis of complex formation of an enzyme with its specific ligands for the first time.
2. The synthetic procedure for a new support (hydrazide derivative of carboxymethyl-cross-linked dextran) in the preparation of biochemically specific adsorbents is also described. The procedure is based on the hydrazidation of a carboxymethylated polysaccharide via its intra-ester (lactone) derivative.
3. The derived support coupled with some compounds containing primary amino group in a good eff iciency.
4. An analysis of the interaction of carboxypeptidase B with its specific ligands was demonstrated with two kinds of its specific adsorbents which were prepared by the new method.
5. Thq demonstration revealed the multiple ligand-binding sites on the enzyme for small molecular ligands and the cooperative interaction of these sites.
