Abstract
It has been reported that a myocardial depressant factor (MDF) accumulates in plasma during various types of circulatory shock. In the present study with dogs subjected to cardiogenic shock, MDF was isolated from the plasma on Bio-gel P-2 column chromatography, and determined under the controlled assay condition. MDF was found to be filtrable, soluble in aqueous media, recovered in the chromatographic fraction of molecular weight range between 800 and 1000, heat stable (i. e. 56°C, 30 min), and to give a positive ninhydrin test. The activity of MDF was shown to be markedly increased in parallel with progression of shock states.
In conclusion, MDF is an important determinant in impairment of myocardial performance during circulatory shock.
