Abstract
Intensive efforts for the standardization of enzyme assay were carried out in many laboratories, and these efforts were resolved themselves into two types of approaches for standardization. One of them is studies of kinetical properties of the enzymes. Another is studies of the relation between enzyme assay conditions and more precise clinical assessment. The first type of approaches were extensively studied in many countries, and GSCC1) and SSCC2) recommended the suitable assay condition for the several enzymes from this point of views. Recently we reported the relation between substrates and some kinetical properties of blood alkaline phosphatases (Al-P)3). In this report, differences of clinical assessment of Al-P in different substrate systems, and the differences of clinical assessment in combination between leucine amino peptidase (LAP) and Al-P in different measuring systems were studied.
Electrophoretically identified three types of sera, bone, hepatic and placental type dominant sera, were used in our experiments.
The assay conditions of Al-P, LAP and Al-P isoenzymes were summarized in TABLE I.
Fig. 1-a shows correlation of Al-P between PP-Carb and PNPP-DEA methods. The regression line with placental type Al-P was different from other sources of Al-P. This fact suggests that the placental type Al-P could be separated from the others by projecting new calculated Z axis. F or this reason, the ratio of Al-P measured with PP-Carb versus Al-P measured with PNPP-DEA were calculated and plotted in Fig. 2-a. From this ratio, placental type Al-P is significantly separated from the others statistically. Fig. 1-b shows correlation of Al-P between PP-Carb and PNPP-Ame method. Three regression lines obtained from the different sources of Al-P were almost identical. The ratio of Al-P measured with PP-Carb and with PNPP-Ame method were also calculated and plotted in Fig. 2-b. From this ratio, there was no difference among the three types of Al-P. So clinical appreciation of Al-P were almost same in this two methods.
For the purpose of elucidating the relation between enzyme assay condition and clinical
