Abstract
A novel sensitive fluorescence reaction of carbohydrates was devised using ethanolamine and borate as reagents. Application of this reagent to the post column labelling of carbohydrates after separation on high performance liquid chromatography provided a simple tool for the detection and determination of reducing sugars in biological samples. This method was also effective in the separation of oligosaccharides. Since this reaction proceeds without strong acid or alkali, the chromatography does not require special equipments. The present method proved to be 100 times as sensitive as refractometric analysis and highly reproducible. This procedure is expected to be useful in the research on the metabolic deficiency of carbohydrates.
