Abstract
A method was developed for fluorescence polarization immunoassay of serum cortisol with cortisol 21-amine, which readily couples with fluorescein isothiocyanate. In this immunoassay, it is not necessary to separate bound and free form. The minimal amount of cortisol detectable was 1.5 ng/tube and serum concentrations of 1.5 to 100μg/dl of cortisol could be measured. A good orrelation was found between the values obtained by this method and by radioimmunoassay. This method is sufficiently sensitive, reproducible, specific, simple and rapid for use in routine determination of serum cortisol.
