Abstract
Guanidino compounds which are one of the uremic toxins are determinated by High Performance Liquid Chromatography (HPLC).
We investigate some conventions to perform with small volume of sample and within short time using HPLC. A length of column is shortened to 1/3 of conventional one, that is 5cm of column length and the analysis of peaks from taurociamine to methylguanidine is shortened to 1/5 (26 min) of conventional one.
A volume of sample used only 1000μl for an analysis. The lineality of standard compounds and precision are also good as well as results with conventional method, so that this method could be used for clinical test.
